A protein has a predicted molecular weight of 47 kDa based on the amino acid sequence, an apparent molecular weight of 50 kDa by SDS-PAGE, and an approximate molecular weight of 95 kDa by size exclusion chromatography.
Question: Part AIn size exclusion chromatography, which of the following proteins would be eluted lastProtein of 28kDProtein of 8kDProtein of 38kDProtein of 18kDRequest AnswerProvide Feedback
Question: in size exclusion chromatography, which of the following proteins would be eluted last Select one A. Protein of 28 kD B. Protein of 8 kD C, Protein of 38 kD D. Protein of 18 kD What is the correct answer choice?
Question: QUESTION 12 A protein with a high percentage of lysine and arginine residues would be BEST purified and concentrated with which type of column (assume a pH=70 buffer)? A. anion exchange OB. affinity chromatography OC reverse-phase chromatography OD size-exclusion chromatography O E. cation exchange
A) cation exchange B) anion exchange C) size-exclusion chromatography D) affinity chromatography E) reverse-phase chromatography 2. A protein with a high percentage of lysine and arginine residues would be BEST purified and concentrated with which type of column?
Question: A protein with a high percentage of lysine and arginine residues would be BEST purified and concentrated with which type of column? O anion exchange O affinity chromatography size-exclusion chromatography O reverse-phase chromatography A ocation exchange
Affinity chromatography Ion-exchange chromatography Size-exclusion chromatography Answer Bank The stationary phase has a covalently bound group to which a protein in the mobile phase can bind.
A mixture of 4 proteins listed below,which should elute 1,2,3&4th in size exclusion chromatography? (a) Cytochrome c Mr 13,000 (b)immunoglobulin G Mr= 145,000 (c) ribonuclease A Mr- 13,700 (d) RNA polymerase Mr= 450,000 47.